Determine The Structural Nature Of This Protein Prelim Mw Determination Gel Fil

Determine the structural nature of this protein:

  1. Prelim MW determination- gel filtration: 1 peak that elutes half way between myosin and B-galactosdase (which is about 158,125Da)
  2. Gel electrophoresis: results are not interpretable
  3. Subunit makeup via gel filtration: 1 peak that elutes about 7% slower than ovalbumin
  4. subunit makeup via SDS PAGE: 1 peak that elutes about 7% faster than ovalbumin
  5. disulfide bonds via gel filtration: 2 peaks, one that elutes with carbonic anhydrase and one that elutes after lysozyme
  6. disulfide bonds via SDS PAGE: 2 peaks, one that migrates the same as carbonic anhydrase and one that migrates faster than lysozyme.
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